Fig. 5
From: Receptors for Advanced Glycosylation Endproducts in Human Brain: Role in Brain Homeostasis
AGEs modulate human fetal astroglia GM-CSF transcription and protein secretion. (A) RT-PCR of GM-CSF mRNA from primary fetal astroglia incubated with 300 µg/ml of FFI-BSA (lanes 1), AGE-BSA (lanes 2), or PMA (62.5 ng/ml) (lanes 3) or medium alone (lanes 4) for 48 hr at 37°C. (B) GM-CSF mRNA levels in peripheral human monocytes cultured under similar conditions with 300 µg/ml FFI-BSA (lanes 1), AGE-BSA (lanes 2), PMA (62.5 ng/ml) (lanes 3), or medium alone (lanes 4). β-actin mRNA was used as internal control. (C) Densitometrie analysis of astrocyte GM-CSF mRNA levels representing four independent experiments (data expressed as % mean ± SD above control cells).