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Fig. 1 | Molecular Medicine

Fig. 1

From: Internalization of a Bacillus anthracis Protective Antigen-c-Myc Fusion Protein Mediated by Cell Surface Anti-c-Myc Antibodies

Fig. 1

Reverse-complement of the 3′ primer used to create PA-c-Myc by PCR amplification of the PA expression vector, pYS5. The top sequence is the 3′ wild-type PA sequence (25). The bottom sequence is the reverse complement of the 3′ PCR primer encoding the 3′ PA sequence, the linker (dashed underlined amino acids), the c-Myc epitope that corresponds to amino acids 410–419 of c-Myc (underlined amino acids) (17), and the unique BamHI restriction site (underlined nucleotides). The capital letters denote the gene sequence and the lowercase letters denote the noncoding vector sequence.

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