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Fig. 4 | Molecular Medicine

Fig. 4

From: Internalization of a Bacillus anthracis Protective Antigen-c-Myc Fusion Protein Mediated by Cell Surface Anti-c-Myc Antibodies

Fig. 4

PA-c-Myc uses c-Myc antibody to enter 9E10 hybridoma cells. PA (A) and PA-c-Myc (B) were serially diluted into 96-well plates in the presence of 100 ng/ml of the LF fusion, FP59, with or without the c-Myc peptide (5.5 µg/ml) as a competitor. Half of the samples also contained the additional competitor PA SNKEΔFF at 0, 5, or 20 µg/ml. After 50–60 hr of toxin challenge, XTT was added to determine cell viability.

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Molecular Medicine

ISSN: 1528-3658