Immunocytochemical localization of the ET-1/AngII receptor in Cosl stable transfectants demonstrating predicted topography and localization to the cell membrane. (A) Control Cosl pMAMneo transfectants show minimal background staining. (B) CoslpMAM-ET-1/AngII transfectants show intense immunostaining with the polyclonal antipeptide antibody raised against the predicted extracellular peptide P51LLTSLGSKE60 (Fig. 1). Primary antibody was reacted with intact cells followed by immunostaining as described in Materials and Methods. Bar corresponds to 32 µM for A and B.