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Fig. 5 | Molecular Medicine

Fig. 5

From: Mechanism of Action of Anti-C1-Inhibitor Autoantibodies: Prevention of the Formation of Stable C1s-C1-inh Complexes

Fig. 5

Measurements of reaction stoichiometry of C1-inh, C1s, and autoantibody. (A) The effect of C1-inh on C1s. The concentration of C1s used in the assay was 23.5 nM and that of C1-inh ranging from 0 to 24.4 nM in a total reaction volume of 3 ml. The half-inhibitory concentration of C1-inh (12.6 nM) is indicated by an arrow. The vertical axis in the plot is the remaining C1s activity calculated according to the enzyme activity assay described in Materials and Methods. (B) The effect of autoantibody on C1-inh. The concentrations of C1s and C1-inh used in the assay were those for half-inhibitory conditions as seen in A and that of autoantibody ranged from 0 to 5 µg in a total reaction volume of 3 ml. The complete inhibitory concentration of autoantibody is .13 nm (arrows). Data shown were obtained with Patient 1 autoantibody but similar results were obtained with Patient 2 autoantibody.

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