Fig. 2

Immunoreactivities for pyrraline, ubiquitin, pentosidine, and neurofilament subunits.

(a) Anterior spinal horn showing scattered axonal spheroids intensely immunostained with anti-pyrraline mAb (×320). (b) A markedly swollen and deformed Betz cell with conglomerate strongly immunostained with the anti-pyrraline mAb and negatively immunostained central vacuoles (×400). (c) Intensely immunoreactive hyaline inclusion for ubiquitin (arrowhead) in neuron soma which is largely immunonegative and moderately immunopositive axonal spheroids (arrow) (×300).(d) Betz cell with conglomerate immunostained with an anti-pentosidine pAb. Note a Betz cell without conglomerate (arrowhead) free of immunoreactivity, serving as internal control (×400). (e) A round conglomerate intensely immunostained for nonphosphorylated neurofilament (NF-np) with a few vacuoles in Betz cells of which perikaryon is moderately immunopositive (×400). (f) Two Betz cells showing strongly immunopositive conglomerates for nonphosphorylated neurofilament light-subunit (NF-L-np) (×300).