Skip to main content

Table 1 Antibody immunoreactivities

From: Advanced Glycation Endproducts in Neurofilament Conglomeration of Motoneurons in Familial and Sporadic Amyotrophic Lateral Sclerosis

Antibody

Source

mAb/pAb

Dilution

Axs

Cgl

MN

HI

Cy/Pia

Makita et al. (30)

mAb

1:24,000

+ 3

+4

+ 1

−

Arg-Lys Imia

Al-Abed and Bucala (31)

pAb

1:900

+ 3

+3

+ 1

−

Pentosidine

Smith et al. (35)

pAb

1:500

+2

+2

+ 1

−

Pyrraline

Smith et al. (35)

mAb

1:300

+ 3

+ 2

+ 1

−

SOD1

Sigma

mAb

1:500

+ 3

+ 3

+ 1

+ 1

Ubiquitin

DAKO

pAb

1:50

+2

+ 1

−

+4

NF-p

SMI

mAb

1:100

+4

+4

−

−

NF-np

SMI

mAb

1:100

+ 3

+4

+2

+ 1

NF-L-np

Zymed

mAb

1:50

+ 3

+4

+ 1

−

b-NOS

Affinity

pAb

1:200

+ 1

+2

−

+ 1

Nitrotyrosine

Beckman et al. (9)

mAb

1:40

+ 1

+2

+ 1

−

  1. Axs, axonal spheroids; Cgl, conglomerates; MN, motor neurons; HI, hyaline inclusions; mAb, monoclonal antibody; pAb, polyclonal antibody; NF-p, phosphorylated neurofilament; NF-np, nonphosphorylated neurofilament; NF-L-np, nonphosphorylated neurofilament light-subunit; b-NOS, brain nitric oxide synthease.
  2. a The anti-cypentodine/piperidine-enolone (Cy/Pi) and anti-arginine-lysine imidazole (Arg-Lys Imi) do not react significantly with pyrraline, pentosidine, or carboxylmethyl-lysine.