Fig. 1

Selective digital subtraction angiograms performed in a patient with critical limb ischemia due to occlusion of all three infrapopliteal vessels at mid-calf level. (A) Immediately prior to, and (B) 1 month post-gene therapy with 2000 µg of naked DNA encoding VEGF. The latter angiogram disclosed a plethora of new collateral vessels in the ischemic limb.