Fig. 1

Representative melting curves of HIV-1 (A) and HCV (B) heteroduplexes used in combinatorial melting assays (left) and representative phylogenetic trees of reference viral sequences (right). Normalized absorbance densities at 490 nm are plotted against temperature measurements for constructed homoduplexes and heteroduplexes of increasing diversity. Normalized absorbance measurements derived for HIV-1 and HCV were normalized by dividing the corresponding relative absorbance measurements (see Table 1) by the highest absorbance value at 70°C for HIV-1 and 69°C for HCV. The error bars represent the standard deviation of multiple capture assays. (A) HIV-1 env duplexes: solid diamond = 0% divergence; solid square = 15% divergence; solid triangle = 30% divergence. (B) HCV C-E1 duplexes: solid diamond = 0% divergence; solid square = 7% divergence; solid triangle = 26% divergence; X = 39% divergence. The numbers next to each of the major phylogenetic “forks” refer to bootstrap values. The error bars represent the standard deviation from independent experiments (n = 5).