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Table 4 Comparative absorbance ratios obtained by COMA and determination of the genetic subtypes of HIV-1 strains

From: Genotyping HIV-1 and HCV Strains by a Combinatorial DNA Melting Assay (COMA)

Name (GenBank accession number)

Subtype

Country Origin

Comparative Absorbance Ratios

Subtype Determined by COMA

   

A

B

C

D

E

F

G

H

 

RO18566a

F

Romania

0.08

0.09

0.02

0.01

0.01

1.00

0.00

0.02

F

TZ940992 (U61878)

A

Tanzania

1.00

0.12

0.08

0.09

0.06

0.09

0.03

0.08

A

MW12203 (L15722)

C

Malawi

0.00

0.00

1.00

0.00

0.00

0.00

0.01

0.00

C

TH942670 (U22587)

B

Thailand

0.01

1.00

0.03

0.00

0.00

0.00

0.00

0.00

B

TH942123 (U22557)

E

Thailand

0.11

0.09

0.05

0.03

1.00

0.03

0.01

0.03

E

MW12199 (L15721)

C

Malawi

0.14

0.07

1.00

0.08

0.15

0.07

0.04

0.10

C

TH942113 (U22625)

E

Thailand

0.00

0.00

0.00

0.00

1.00

0.00

0.00

0.31

E

TH942711 (U22560)

B

Thailand

0.04

1.00

0.03

0.01

0.01

0.00

0.00

0.00

B

KE943382a

D

Kenya

0.02

0.00

0.01

1.00

0.00

0.00

0.00

0.00

D

KE943509a

A

Kenya

1.00

0.16

0.07

0.09

0.07

0.14

0.03

0.06

A

UG5428a

D

Uganda

0.09

0.03

0.01

1.00

0.00

0.01

0.00

0.00

D

  1. The comparative absorbance ratios were determined as explained in Materials and Methods using the subtype-reference pools described in Table 2. HIV-1 genetic subtypes had been previously determined by phylogenetic analyses of nucleotide sequences. aUnpublished sequences communicated by M. L. Kalish. For each unknown sample, the reference pool which produces the highest relative absorbance (comparative absorbance ration = 1.00), shown in bold numbers, indicates the correct genetic subtype. Hybridization temperature was 76 ± 0.5°C.