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Fig. 6 | Molecular Medicine

Fig. 6

From: Classification of Breast Cancer Cells on the Basis of a Functional Assay for Estrogen Receptor

Fig. 6

Hormone dependency of activation of transfected PS2 promoter. ER+ MCF-7 and ER+21 FT cells were grown in 25 ml of complete medium (designated by FBS) in 150-mm tissue culture dishes. After 24 hr the medium was replaced with either complete medium (FBS) or medium containing stripped serum (DCC). Transfection by DEAE dextran of cells with fusion plasmid PS2-CAT (10 µg) was as described previously (20,37). Forty-eight hours after transfection the cells were treated with indicated concentrations of E2 and 2 hr later cell extracts were prepared. As controls, cells were also co-transfected with pSV-lacZ. β-galactosidase activity was the same in the extracts of control and E2-treated transfected cells, suggesting that the hormone treatment did not influence an SV40 promoter that lacks the ERE sequence. Panels A and B show the results of transfection experiments with MCF-7 and 21PT cells, respectively.

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