Fig. 1
From: Subtyping Analysis of Fanconi Anemia by Immunoblotting and Retroviral Gene Transfer
Screening of Fanconi anemia lymphoblast and fibroblast lines for expression of FAA and FAC proteins. (A) EBV-immortalized lymphoblasts from FA patients were screened by immunoblot with either anti-FAA (upper blot) or anti-FAC (lower blot). Cell lines tested were HSC72 (lane 1), HSC72 after pMMP-FAA transduction (lane 2), HSC536 (lane 3), HSC536 after pREP4-FAC transfection (lane 4), PD4510 (lane 5), PD153 (lane 6), PD4 (lane 7), HSC62 (lane 8), PD20 (lane 9), PD36 (lanes 10, 20), PD289 (an unknown type, not included in this analysis, presumed to be type A on the basis of a truncation mutant detected with an anti-FAA carboxyl-terminal antibody, lane 11), VU 337 (lane 12), VU 373 (lane 13), EUFA 007 (lane 14), EUFA 275 (lane 15), EUFA 444 (lane 16), EUFA 445 (lane 17), EUFA 471 (lane 18), PD9, (lane 19), PD45 (lane 21), PD56 (lane 22), and PD113 (lane 23). Possible truncated FAA proteins were observed in lanes 12 and 22 (arrowheads). (B) Primary fibroblasts from FA patients were analyzed by immunoblot with either an anti-FAA antiserum (upper panel) or an anti-FAC antiserum (lower panel). Cell lines analyzed were PD426 (lane 1), PD123 (lane 2), PD303 (lane 3), PD320 (lane 4), PD473 (lane 5), PD289 (lane 6), and PD7 (normal control, lane 7).