Immunoelectron microscopy of AGEs in cataractous lenses obtained from smokers and nonsmokers. Processing of lenses for electron microscopy is described in Materials and Methods. (A, B) Lens sections from nondiabetic nonsmokers. (C, D) Lens sections from nondiabetic smokers probed with the polyclonal anti-AGE-RNase A serum. (E, F) Sections from nondiabetic smokers probed with an anti-RNase A control serum (reacting only with the AGE-carrier protein). Magnification, A, C, E: ×9000; B, D, F: × 15,000.