Fig. 3From: Requirements for Enhanced Transgene Expression by Untranslated Sequences from the Human Cytomegalovirus Immediate-Early GeneAddition of 5′ sequences from CMV IE gene is associated with an increase in steady-state mRNA. Northern analysis of RNA from 293 cells transfected with pCMVintCAT (lane 2) or pCMVCAT (lane 3) with nontranfected 293 cells (lane 1). The upper band (1.6 kb) (dotted arrow) is the predominant band from pCMVintCAT transfections compared with the expected 0.7 kb band (solid arrow) also seen in the pCMVCAT transfections consistent with CAT mRNA.Back to article page