Fig. 2
From: Involvement of Macrophage Migration Inhibitory Factor (MIF) in the Mechanism of Tumor Cell Growth
Expression of MIF protein in colon 26 cells and transfected cells. Western blot analysis was carried out to examine the expression of MIF protein by each cell type. The transfected bands were visualized by a Konica HRP-1000 immunostaining kit. Lane 1, molecular weight markers; lane 2, recombinant rat MIF (100 ng/ml); lane 3, colon 26 cells (2 × 105 cells); lanes 4–6, colon 26 cells (2 × 105 cells) transfected with pBK-MIFAS; lanes 7–9, colon 26 cells (2 × 105 cells) transfected with pBK vector alone.