Fig. 1

Deduced amino acid sequence alignment of Pvs25 and Pvs28 from Plasmodium vivax with the consensus sequence of P25 and P21/25 subfamily members. The sequences of Pvs25 and Pvs28 are shown in uppercase and have been arranged in six lines, representing the secretory signal sequence, the four EGF-like domains, and the C-terminal hydrophobic region, respectively. The cysteine residues that comprise the EGF-like motifs were aligned with the consensus amino acid sequences of zygote/ookinete surface proteins of other malaria parasites: Pys25 (8), Pbs25 (10), Pfs25 (4), and Pgs25 (6) in the P25 subfamily, and Pys21 (8), Pbs21 (11), Pfs28 (5), and Pgs28 (7) in the P21/28 subfamily. The amino acid sequences were deduced from universal codon usage and manually aligned with previously published deduced amino acid sequences. Consensus represents the consensus amino acid sequence in all of these proteins. P25con represents the consensus amino acid sequence in the P25 subfamily members. P28con represents the consensus amino acid sequence in the P21/28 subfamily members. Dots in the consensus amino acid sequences are residues nonidentical to the sequences in each group. Nucleotide sequence data of Pvs25 and Pvs28 derived from Plasmodium vivax Sal I are available in the Gen-Bank, EMBL, and DDBJ databases under the accession numbers Pvs25, AF083502 and Pvs28, AF083503.