Fig. 3

In situ hybridization analysis of embry-oid bodies.

Shown is in situ hybridization detection of expression of neurofilament 68Kd subunit (NF), ζ-globin (ζGlob), α-fetoprotein (αFP) and α-cardiac actin (cAct) in 20-day-old EBs. Shown are 5 µm paraffin-embedded serial sections of three different EBs stained with hematoxylin and eosin (H&E) or hybridized to specific 5′-biotinilated RNA probes and labeled with a fluorogenic product of streptavidin-conjugated alkaline phosphatase (16). Con-hybridization with nonspecific RNA. Scale bar, 100 µm.