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Fig. 4 | Molecular Medicine

Fig. 4

From: Differentiation of Human Embryonic Stem Cells into Embryoid Bodies Comprising the Three Embryonic Germ Layers

Fig. 4

Cardiac muscle differentiation depicted in a pulsing embryoid body.

(A) Contracting em-bryoid body. Shown is a video micrograph of a pulsing EB in a relaxed or contracted state. Light absorption in the video micrographs was color coded in either green or red; the two photos were overlaid to demonstrate the changes in opacity, reflecting contraction during the pulsation of the EB. Scale bar, 200 µm. (B) Rhythmic contraction. Presented is the magnitude of contraction recorded from the pulsing EB over 20 sec. (C) Layered cardiac muscle cells. Shown are serial sections from the pulsing EB, including in situ hybridization depicting expression of α-cardiac actin (cActin) in it. Also shown is a section stained with hematoxylin and eosin (H&E) and a section hybridized with nonspecific RNA (control). Scale bar, 100 µm.

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