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Fig. 1 | Molecular Medicine

Fig. 1

From: Immunological Evidence that Non-carboxymethyllysine Advanced Glycation End-products Are Produced from Short Chain Sugars and Dicarbonyl Compounds in vivo

Fig. 1

Characteristics of rabbit serum albumin incubated with glyceraldehyde, glycolaldehyde, methylglyoxal and glyoxal (AGE-RSAs).

Rabbit serum albumin (20 mg/ml) was incubated under sterile conditions with 0.1 M D-glyceraldehyde, glycolaldehyde, methylglyoxal or glyoxal and 5 mM DTPA in 0.2 M phosphate buffer (pH 7.4) at 37°C for 7 d and then low molecular weight reactants and aldehydes were removed using a PD-10 column and dialysis against phosphate-buffered saline (PBS, pH 7.4). (A) Fluorescence emission spectra of AGE-RSAs (1.0 mg/ml) at 360 nm after incubation with glyceraldehyde (Δ), glycolaldehyde (▲), methylglyoxal (□), glyoxal (■) or glucose (), and after incubation without these compounds (). The incubation time was 7 d, except for glucose (8 weeks). (B) SDS-PAGE of AGE-RSAs. Two micrograms of nonglycated RSA (without compounds, lane 1) and AGE-RSAs (incubated with glucose (lane 2), glyceraldehyde (lane 3), glycolaldehyde (lane 4), methylglyoxal (lane 5) and glyoxal (lane 6) were loaded onto a 7.5% polyacrylamide gel. Staining of the gel was done with Coomassie Brilliant Blue. Size markers (kD) are shown on the left.

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