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Fig. 4 | Molecular Medicine

Fig. 4

From: Immunological Evidence that Non-carboxymethyllysine Advanced Glycation End-products Are Produced from Short Chain Sugars and Dicarbonyl Compounds in vivo

Fig. 4

Immunoblot analysis of various AGE-proteins with non-CML AGE antibodies.

Aliquots (0.5 µg) of AGE-BSAs (incubated with glucose (lane 1), glyceraldehyde (lane 2), glycolaldehyde (lane 3), methylglyoxal (lane 4), and glyoxal (lane 5)) were loaded onto a 7.5% polyacrylamide gel. Formation of non-CML AGE in the protein was determined by immunoblot analysis using affinity-purified non-CML AGE antibodies. Non-CML AGE antibody was obtained by incubation of AGE-RSA with glyceraldehyde (A), glycolaldehyde (B), methylglyoxal (C) and glyoxal (D). Size markers (kD) are shown on the left.

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Molecular Medicine

ISSN: 1528-3658