Fig. 6

Immunoblot analysis of serum from diabetic patients on hemodialysis with non-CML AGE antibodies.

20 µg of serum was treated with 1%-SDS in the absence of 2-mercaptoethanol and the sample was loaded onto 5–20% gradient polyacrylamide gel. (A) Detection of serum protein bands was performed with Coomassie Briliant Blue staining. (B) Formation of non-CML AGE was determined by immunoblot analysis using affinity-purified non-CML AGE antibodies. Non-CML AGE antibody was obtained by incubation of AGE-RSA with glyceraldehyde (lane 1), glycolaldehyde (lane 2), methylglyoxal (lane 3) and glyoxal (lane 4). Size markers (kD) are shown on the left.