Fig. 7
From: A Secreted Tumor-Suppressor, mac25, with Activin-Binding Activity
Binding of mac25 and activin A.
(A) Proteins in lysates and media after immunoprecipitation with antibody against GFP (α GFP) or activin A (αactivin). Saos-2 cells were transfected with the control vector (Saos-2 GFP) or the mac25-GFP fusion construct (Saos-2 mac25GFP) and proteins were probed with antibody against activin A. Activin A was detected after precipitation with antibody against GFP in all samples except those derived from the lysate of Saos-2 GFP. (B) The same lysates and media were probed with antibody against GFP. The mac25-GFP fusion protein was detected in all samples of Saos-2 mac25GFP. The GFP protein was detected in lysate of Saos-2 GFP after precipitation with antibody against GFP, but it was not detected in lysate of Saos-2 GFP after precipitation with antibody against activin A.