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Fig. 8 | Molecular Medicine

Fig. 8

From: Induction of Placental Heme Oxygenase-1 Is Protective Against TNFα-induced Cytotoxicity and Promotes Vessel Relaxation

Fig. 8

Hemin mediated carbon monoxide release. Tissue and cells were exposed to hemin and carbon monoxide levels determined as a measure of the percentage of carboxyhemoglobin (COHb) in the media. (A) First-trimester trophoblasts were exposed to 5 µM hemin for up to 24 hr. (B) The effect of HO inducer and HO inhibitor on CO production in placental explants. Tissue was exposed to 20 µM SnPP (HO inhibitor) for 1 hr and then incubated with 5 µM hemin for 2 hr. An inset shows the effect of hemin (5 µM) on CO production from term villous fragments over a 24-hr period. (C) Fragments of chorio-decidua were exposed to 5 µM hemin for up to 24 hr. CO release is expressed as a percentage of carboxyhemoglobin (%COHb) in the conditioned media and standardized per mg/ml of total protein. Data are shown as the mean ± S.E.M. of three separate experiments performed in triplicate. *p < 0.01 vs hemin; **p < 0.001 vs vehicle.

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