Fig. 2
Expression of anti-MA Fab3H7 fragments in a rabbit reticulocyte lysate system
[35S]-cysteine-labeled proteins were synthesized in rabbit reticulocyte lysate and were analyzed by electrophoresis on a 12.5% SDS-PAGE gel. The gel is a 4-hr autoradiograph. Lane 1: pRc/CMV plasmid expressing anti-MA Fab3H7 fragments with native immunoglobulin leader sequences; lane 2: pRc/CMV plasmid expressing anti-MA Fab3H7 fragments without native immunoglobulin leader sequences; lane 3: pRc/CMV control plasmid; lane 4: luciferase cDNA as T7 positive control. Arrows indicate Fd heavy and light chains.