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Fig. 3 | Molecular Medicine

Fig. 3

From: Inhibition of Early and Late Events of the HIV-1 Replication Cycle by Cytoplasmic Fab Intrabodies against the Matrix Protein, p17

Fig. 3

Immunoprecipitation of Fab3H7 fragments from stably transfected COS-1 cells

[35S]-cysteine-labeled proteins from the cell lysate or culture medium of parental COS-1 cells. COS-pRc/CMV-vector, or COS-Fab3H7+L cells were immunoprecipitated with rabbit anti-mouse IgG and were analyzed by electrophoresis on a 12.5% SDS-PAGE gel. Lanes 1, 3, 5: cell lysate; lanes 2, 4, 6: cell supernatant. Lanes 1 and 2: parental COS-1 cells; lanes 3 and 4: COS-pRc/CMV-vector cells; lanes 5 and 6: COS-Fab3H7+L cells (clone 15/72). Arrows indicate Fd heavy and light chains

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Molecular Medicine

ISSN: 1528-3658