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Fig. 4 | Molecular Medicine

Fig. 4

From: Inhibition of Early and Late Events of the HIV-1 Replication Cycle by Cytoplasmic Fab Intrabodies against the Matrix Protein, p17

Fig. 4

Secretion of functional anti-MA Fab3H7 fragments from stable CD4 + Jurkat T cells

Fresh medium from individual stable Jurkat-Fab3H7 + L cell clones were harvested after 24 hr incubation, concentrated 10Ă— in an Amicon concentrator (Beverly, MA), and added to ELISA wells in triplicate that were coated with recombinant HIV-1 MA (Agmed) at 100 ng/well. Binding of Fab3H7 fragments was detected by HRP-labeled rabbit anti-mouse IgG as the secondary antibody and the standard deviations were <5%. Supernatants from the following sources were tested: Lane 1: 3H7 hybridoma cells; lane 2: Jurkat-pRc/CMV-vector cells; lane 3: stable Jurkat-Fab3H7 + L subclone 15/72-6.

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