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Fig. 5 | Molecular Medicine

Fig. 5

From: Inhibition of Early and Late Events of the HIV-1 Replication Cycle by Cytoplasmic Fab Intrabodies against the Matrix Protein, p17

Fig. 5

Radioimmunoprecipitation of cytoplasmic anti-MA Fab3H7 intrabody fragments

[35S]-cysteine-labeled proteins from cell lysates of stably transfected COS-1 cells were immunoprecipitated with rabbit anti-mouse IgG and were analyzed by electrophoresis on a 12.5% SDS-PAGE gel. Lane 1: molecular weight standards; lane 2: parental COS-1 cells; lane 3: COS-Fab3H7 + L cells (clone 15/62); lane 4: COS-Fab3H7-L cells (clone 621); lane 5: COS-pRc/CMV-vector cells. Arrows indicate Fd heavy and light chains.

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