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Fig. 6 | Molecular Medicine

Fig. 6

From: Inhibition of Early and Late Events of the HIV-1 Replication Cycle by Cytoplasmic Fab Intrabodies against the Matrix Protein, p17

Fig. 6

Inhibition of CAT activity in stably transfected CD4 + Jurkat T cells expressing cytoplasmic Fab3H7 intrabodies following infection with HIV-1 CAT virus

Seventy-two hours post-infection with 10,000 cpm/ml RT activity (pHXBΔenvCAT/pSVIIIenv), cell lysates were prepared and CAT activity was determined. (A) Lane 1: Jurkat parental cells; lane 2, Jurkat-pRc/CMV-vector cells; lane 3: Jurkat-Fab3H7-L cells (clone 621–3); lane 4: Jurkat-Fab3H7-L cells (clone 725–3); lane 5: (+) control, CAT enzyme; lane 6: (−) control, no CAT enzyme. (B) Lane 1: Jurkat parental cells; lane 2: Jurkat FdA3X8/K3H7 cells; lane 3: Jurkat-Fab-3H7-L cells (clone 621–3); lane 4: Jurkat-Fab3H7-L cells (clone 725–3); lane 5, (+) control, CAT enzyme; lane 6, (−) control, no CAT enzyme.

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Molecular Medicine

ISSN: 1528-3658