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Fig. 7 | Molecular Medicine

Fig. 7

From: Inhibition of Early and Late Events of the HIV-1 Replication Cycle by Cytoplasmic Fab Intrabodies against the Matrix Protein, p17

Fig. 7

Inhibition of HIV-1 infection in stably transfected CD4 + Jurkat T cells expressing cytoplasmic anti-MA Fab3H7 intrabodies

Subclones of CD4+ Jurkat-Fab3H7-L cells (1 × 106) were infected with either of two isogeneic laboratory strains of HIV-1 that differed in the presence or absence of the accessory gene, vpu or two European SI primary isolates. Cell-free p24 levels were recorded on the indicated days. (A) Infection with HXIIIBvpu+ at 2000 cpm/ml RT activity (MOI 0.5) of Jurkat-pRc/CMV-vector cells (), Jurkat-Fab3H7 (clone 621–1) (), Jurkat-Fab3H7 (clone 621–2) (□), and Jurkat-Fab3H7 cells (clone 621–3) (). (B) Infection with HXIIIBvpu−. at 15,000 cpm/ml RT activity (MOI6.5) of Jurkat-pRc/CMV-vector cells (), Jurkat-Fab3H7 (clone 621–3) (), and Jurkat-Fab3H7 cells (clone 725–3) (). (C and D) Parental Jurkat cells (), Jurkat-vector cells (), and Jurkat-Fab3H7 cells (clone 725–3) () (1 × 106) were infected with 25 ng p24 of two different European, syncytia-inducing (SI) primary isolates that were previously screened for their ability to infect parental CD4+ Jurkat T cells. (C) Infection with primary isolate #1. (D) Infection with primary isolate #2. (E) Infection with HIV-2NIH-Z or HXBIIIBvpu−. (MOI 0.5). Open symbols represent infection with HXIIIBvpu−; solid symbols, infection with HIV-2NIH-Z; circles, Jurkat-vector cells; and triangles, Jurkat-Fab3H7 cells (clone 723–5).

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