Fig. 8
HIV-1 DNA integration in Jurkat control and Jurkat-Fab3H7-L cells from an experiment similar to that in Fig. 7B
Lane 1: HaeIII digested ϕX174 DNA molecular weight standards; lane 2: uninfected Jurkat cells; lanes 3, 6, 9: infected Jurkat cells; lanes 4, 7, 10: infected Jurkat-Fab3H7-L cells (clone 621–3); lanes 5, 8, 11: infected Jurkat-Fab3H7-L cells (clone 725–3). Lanes 2–5: Day 4; lanes 6–8: Day 10; lanes 9–11: Day 13; lane 12, uninfected Jurkat cells spiked with 1–3.5 kb fragments from SacI/XbaI digested HXBc2 plasmid that contains the SVCvpu+. HIV-1 provirus; lane 13: PCR primers alone, no cellular DNA; lane 14: same as lane 12 but spiked with proviral DNA fragments <1 kb.