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Fig. 3 | Molecular Medicine

Fig. 3

From: The Cytoplasmic Domain of Alzheimer’s Amyloid Precursor Protein Is Phosphorylated at Thr654, Ser655, and Thr668 in Adult Rat Brain and Cultured Cells

Fig. 3

Specificity of phosphorylation state-specific antibodies towards enzymatically phosphorylated APP 645–694

APP645–694 (6 µg) was incubated with nonradioactive ATP and CaM kinase II (CaMk), PKC, or without enzymes (−). Samples were subjected to SDS-PAGE, and transferred to nitrocellulose membranes. Membranes were probed with pAbT654 (lanes 1 to 3), pAbS655 (lanes 4 to 6), or G-369 (lanes 7 to 9) antibodies, followed by [125I]-protein A, and autoradiography was performed. The arrow indicates the position of dephospho-APP645–694.

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Molecular Medicine

ISSN: 1528-3658