Table 2 Primer pairs for amplification of LAMC2 cDNA sequences by RT-PCR
From: Detection of Novel LAMC2 Mutations in Herlitz Junctional Epidermolysis Bullosa
Primer | Positiona | Sequence | Annealing Temperature (°C) | Product Size (bp) |
|---|---|---|---|---|
1L | 35 | 5′-GAGCGCAGAGTGAGAACCAC-3′ | 60 | 691 |
1R | 726 | 5′-ACTGTATTCTGCAGAGCTGC-3′ |  |  |
2L | 706 | 5′-CGCAGCTCTGCAGAATACAG-3′ | 60 | 439 |
2R | 1145 | 5′-AGATTCCGCAGTAACCTTCG-3′ |  |  |
3L | 1046 | 5′-GGCTCACCAAGACTTACACA-3′ | 60 | 491 |
3R | 1537 | 5′-GAATCACTGAGCAGCTGAAC-3′ |  |  |
4L | 1430 | 5′-CTGACATTGAGTGTGCTGAC-3′ | 60 | 453 |
4R | 1883 | 5′-CCATCACTTCGACATCCTAC-3′ |  |  |
5L | 1854 | 5′-CTCAGAGCCTGTAGGATGTC-3′ | 60 | 501 |
5R | 2355 | 5′-GTCTGAGGCAGGAATGTTAG-3′ |  |  |
6L | 2248 | 5′-CAGTACCAGAACCGAGTTCG-3′ | 60 | 529 |
6R | 2777 | 5′-CTGGTTACCAGGCTTGAGAG-3′ |  |  |
7L | 2674 | 5′-GATTCAGTGTCTCCGCTTCA-3′ | 56 | 539 |
7R | 3213 | 5′-GGCTTCCAAGTTCAGACTCC-3′ |  |  |
8L | 3168 | 5′-CTCCAGTGAGATTGAACAGG-3′ | 62 | 534 |
8R | 3702 | 5′-GCTTCACTGTTGCTCAAGAG-3′ |  |  |