TNFbp treatment inhibits glomerular and tubular MIF expression and macrophage accumulation in rat anti-GBM glomerulonephritis
(a) Quantitation of glomerular MIF mRNA expression by in situ hybidization; (b) quantitation of glomerular MIF protein expression by immunohisto-chemistry; (c) quantitation of glomerular ED 1 -positive macrophages; (d) quantitation of tubular MIF mRNA expression by in situ hybidization; (e) quantitation of tubular MIF protein expression by immunohisto-chemistry; and (f) quantitation of ED 1 -positive macrophages within the tubulointerstitium. Saline-treated animals are represented by solid lines and closed circles, TNFbp-treated animals, by dotted lines and open circles. Data are expressed as the mean ± SEM from groups of six animals. *p < 0.05, **p < 0.01, and ***p < 0.001, compared with saline-treated animals.