TNFbp treatment inhibits glomerular and tubular MIF expression and macrophage accumulation in rat anti-GBM glomerulonephritis
Combined in situ hybridization and immunohistochemistry show MIF mRNA expression (blue) by intrinsic kidney cells and by EDI-positive macrophages (brown) on Day 7 of disease with (a) saline or (b) TNFbp treatment. Double immunohistochemistry shows colocalization of strong MIF expression (blue) and ED1-positive macrophages (brown) on Day 14 of disease with (c) saline or (d) TNFbp treatment. Macrophages (brown) expressing MIF mRNA or protein are indicated by arrowheads. Strong MIF expression and the development of crescent formation and tubulitis are seen. Sections were counterstained with PAS minus haematoxylin. Original magnification, ×400.