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Fig. 2 | Molecular Medicine

Fig. 2

From: Normal Human Epidermal Keratinocytes Express In Vitro Specific Molecular Forms of (Pro)Filaggrin Recognized by Rheumatoid Arthritis-Associated Antifilaggrin Autoantibodies

Fig. 2

Double indirect immunoflorescence analysis of cultured human keratinocytes with AKH1, a MAb to (pro)filaggrin, and an RA serum

The labeling of AKH1 is revealed with tetramethylrhodamine isothiocyanate-conjugated anti-mouse IgG (A, C) and that of the RA serum with fluorescein isothiocyanate-conjugated anti-human IgG (B, D). The MAb and the RA serum label the same granules (arrows). Dotted lines denote the basal limit of the cultured epithelial sheets. Bar = 200 µm (A, B); 50 µm(C, D).

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Molecular Medicine

ISSN: 1528-3658