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Fig. 4 | Molecular Medicine

Fig. 4

From: Normal Human Epidermal Keratinocytes Express In Vitro Specific Molecular Forms of (Pro)Filaggrin Recognized by Rheumatoid Arthritis-Associated Antifilaggrin Autoantibodies

Fig. 4

Immunoblotting reactivity of RA serum and MAbs AHF1 and AHF7 on low-salt extracts of cultured keratinocytes and normal human epidermis separated by two-dimensional isoelectrofocusing/SDS-PAGE

In the extracts from cultured keratinocytes (upper half), the RA serum and the AHF monoclonal antibodies stain a series of antigens exhibiting an S-shaped migration pattern (pI ranging from 5.5 to 7.3 and molecular weights ranging from 40 up to >200 kDa). In the extracts from normal epidermis (lower half), the RA serum and the AHF MAbs stain the comma-shaped neutral/acidic isoform of filaggrin (pI ranging from 6 to 7.4 and molecular weights ranging from 37 to 40 kDa). Molecular weight markers are on the left, pI markers are on the top of each membrane. IEF, isoelectrofocusing.

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Molecular Medicine

ISSN: 1528-3658