Immunohistochemical localization of mutant PV protein in cells of liver
Liver from a NTG mouse (A, C, E) and a TG mouse from family D (B, D, F) using mouse monoclonal antibody C4 (anti-wild type TRβl) (C, D), rabbit polyclonal antibody Tl (anti-PV mutant TRβl) (E, F) or a blank control deleting the first antibody step (A, B). The antimouse IgG conjugate (A–D) reacts with endogenous mouse IgG present in the sinusoidal spaces of the mouse liver (shown by arrowheads in A and B). Hepatocyte nuclei that contain TR are not easily detected in the blank control (A and B, arrows) because the levels of background labeling in the nuclei were low, allowing detection of specific TR localization as shown in C and D (arrows) by using anti-wild-type TRβl C4. When anti-PV antibody Tl is used, no specific nuclear labeling is detected in NTG hepatocytes (E, arrow), whereas hepatocyte nuclei from the TG mouse demonstrate easily detectable labeling (F, arrow). (×370, bar = 10 µm).