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Table 3 AIDS-KS cell growth inhibition by IL-4 and IL-4(38-37)-PE38KDEL in clonogenic assay

From: Interleukin-4 Receptor Expression on AIDS-Associated Kaposi’s Sarcoma Cells and Their Targeting by a Chimeric Protein Comprised of Circularly Permuted Interleukin-4 and Pseudomonas Exotoxin

  Colonies ± S.D. (% of control)
  KS-248 (AIDS-KS) NCB-59 (AIDS-KS) HUVEC (normal endothelial)
IL-4 (ng/ml)
1 82 ± 1.9 89 ± 1.9 89 ± 5.5
10 79 ± 1.9 79 ± 3.6 79 ± 6.1
100 78 ± 5.7 75 ± 2.4 82 ± 2.5
1000 73 ± 4.1 66 ± 1.5 80 ± 1.5
IL-4(38-37)PE38KDEL (ng/ml)
1 71 ± 5.3 65 ± 3.0 97 ± 7.7
5 32 ± 2.3 49 ± 2.8 74 ± 6.0
50 5 ± 1.2 10 ± 0.6 65 ± 3.3
100 0 0 66 ± 7.2
200 0 0 51 ± 7.1
  1. KS248 or NCB-59 (500 cells of each) and HUVECs (200 cells) were plated in triplicate in 100-mm or 60-mm petri dishes, respectively, and treated with either IL-4 or IL-4 toxin on the subsequent day. The culture dishes were incubated for 8 to 10 days and then washed with PBS and colonies stained with crystal violet. The colonies consisting of >50 cells were scored. The results are presented as a percentage of the colony counts in control and treated groups.