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Table 3 AIDS-KS cell growth inhibition by IL-4 and IL-4(38-37)-PE38KDEL in clonogenic assay

From: Interleukin-4 Receptor Expression on AIDS-Associated Kaposi’s Sarcoma Cells and Their Targeting by a Chimeric Protein Comprised of Circularly Permuted Interleukin-4 and Pseudomonas Exotoxin

 

Colonies ± S.D. (% of control)

 

KS-248 (AIDS-KS)

NCB-59 (AIDS-KS)

HUVEC (normal endothelial)

IL-4 (ng/ml)

1

82 ± 1.9

89 ± 1.9

89 ± 5.5

10

79 ± 1.9

79 ± 3.6

79 ± 6.1

100

78 ± 5.7

75 ± 2.4

82 ± 2.5

1000

73 ± 4.1

66 ± 1.5

80 ± 1.5

IL-4(38-37)PE38KDEL (ng/ml)

1

71 ± 5.3

65 ± 3.0

97 ± 7.7

5

32 ± 2.3

49 ± 2.8

74 ± 6.0

50

5 ± 1.2

10 ± 0.6

65 ± 3.3

100

0

0

66 ± 7.2

200

0

0

51 ± 7.1

  1. KS248 or NCB-59 (500 cells of each) and HUVECs (200 cells) were plated in triplicate in 100-mm or 60-mm petri dishes, respectively, and treated with either IL-4 or IL-4 toxin on the subsequent day. The culture dishes were incubated for 8 to 10 days and then washed with PBS and colonies stained with crystal violet. The colonies consisting of >50 cells were scored. The results are presented as a percentage of the colony counts in control and treated groups.
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Molecular Medicine

ISSN: 1528-3658