| |
Colonies ± S.D. (% of control)
|
|---|
| |
KS-248 (AIDS-KS)
|
NCB-59 (AIDS-KS)
|
HUVEC (normal endothelial)
|
|---|
|
IL-4 (ng/ml)
|
|
1
|
82 ± 1.9
|
89 ± 1.9
|
89 ± 5.5
|
|
10
|
79 ± 1.9
|
79 ± 3.6
|
79 ± 6.1
|
|
100
|
78 ± 5.7
|
75 ± 2.4
|
82 ± 2.5
|
|
1000
|
73 ± 4.1
|
66 ± 1.5
|
80 ± 1.5
|
|
IL-4(38-37)PE38KDEL (ng/ml)
|
|
1
|
71 ± 5.3
|
65 ± 3.0
|
97 ± 7.7
|
|
5
|
32 ± 2.3
|
49 ± 2.8
|
74 ± 6.0
|
|
50
|
5 ± 1.2
|
10 ± 0.6
|
65 ± 3.3
|
|
100
|
0
|
0
|
66 ± 7.2
|
|
200
|
0
|
0
|
51 ± 7.1
|
- KS248 or NCB-59 (500 cells of each) and HUVECs (200 cells) were plated in triplicate in 100-mm or 60-mm petri dishes, respectively, and treated with either IL-4 or IL-4 toxin on the subsequent day. The culture dishes were incubated for 8 to 10 days and then washed with PBS and colonies stained with crystal violet. The colonies consisting of >50 cells were scored. The results are presented as a percentage of the colony counts in control and treated groups.
