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Fig. 4 | Molecular Medicine

Fig. 4

From: Induction of Aquaporin-1 mRNA following Cardiopulmonary Bypass and Reperfusion

Fig. 4

AQP1 mRNA in lung before and after CPB/HCA followed by reperfusion

(A) Autoradiograph of RNase protection assay. Each lane represents a separate tissue specimen; each condition represents 2–3 animals. Control animals underwent sternotomy only for 20 or 260 min. Reperfusion animals underwent CPB/HCA (2 hr of circulatory arrest at 15°C) followed by 0, 3, or 6 hr of reperfusion at 37°C The non-muscle actin-protected band is 63 nucleotides (nt). Three fragments of AQP1 mRNA are seen below the expected 125 nt AQP1-protected band. (B) Quantitation of AQP1 mRNA levels obtained from PhosphorImager analysis of the sequencing gel in A. Background was determined by tRNA control. AQP1 was normalized to the internal actin control, [AQP1-tRNA]/[actin-tRNA]. All samples were then normalized to a mean of 1 for the 20-min control samples. Comparative values of AQP1 normalized to actin were similar, regardless of whether the single 125-nt AQP1 band or all 4 bands were quantitated.

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