Fig. 4
From: Induction of Skin Fibrosis in Mice Expressing a Mutated Fibrillin-1 Gene
Reverse transcription-polymerase chain reaction (RT-PCR) analysis of the expression of pdFbn-1 gene. 10 µg RNA extracted from lung or kidney or fibroblast lines was used to synthesize single-strand DNA, according to the method described in “Material and Methods.” lane 1: Φx174/HaeIII; lane 2: lung cDNA from high copy number transgenic (Tg) mouse; lane 3: lung cDNA from low copy number Tg mouse; lane 4: lung cDNA from C57Bl/6 mouse; lane 6: lung cDNA from tight skin (TSK)/+ mouse; lane 7: cDNA from low copy number Tg fibroblast line; lane 8: cDNA from low copy number Tg fibroblast line; lane 9: cDNA from C57BL/6 fibroblast line; line 9: cDNA from TSK/+ fibroblast line; lane 10: no cDNA. Low copy number Tg mice were obtained from breeding of C57Bl/6 mice with founder TS4-1 and high copy number Tg mice with founder TS4-3 or TS4-4 mice. pdFbn-1, plasmid bearing fibrillin-1 gene.