Fig. 4

Characterization of the anti-presenilin 1 (PS1) mAb (NT1) by Western blot analysis. (A) Proteins were prepared as in Figure 2 and probed with NT1. The following were loaded onto the gel: 2 ng glutatheone S-transferase fusion protein contract (GST-PS1/NT; lane 1), 50 ng GST (lane 2), 50 µg total protein extracted from murine L cells overexpressing human PS2 (L/PS2, lane 3) or human PS1 (L/PS1, lane 4), and 200 µg protein prepared from human prefrontal cortex (PFC) as described in “Materials and Methods” (lanes 5–10). Lanes 8 and 9 were probed with NT1 in the presence of peptide B; lane 10, with the GST-PS2/NT fusion protein. Specificity of PS1 detection in human brain. Serial 40 µm-vibratome sections from human prefrontal cortex were processed for immunocytochemistry as described in “Materials and Methods” and incubated with either NT1 (B) or NT1 in the presence of peptide B (C). Similar serial sections were incubated in P1 (D) or P1 in the presence of peptide C (E).