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Table 1 Subpopulation and cell cycle profiles for chronic myeloid leukaemia (CML) and normal bone marrow (NBM) CD34+Lin− stem cells

From: The Effect of Bcr-Abl Protein Tyrosine Kinase on Maturation and Proliferation of Primitive Haematopoietic Cells

Sample % of CD34+Lin- % of cells in cycle
Thy+Rh lo Thy+ Rh hi Thy-Rh lo Thy-Rh hi Thy+ Rh lo Thy+ Rh hi Thy-Rh lo Thy-Rh hi
NBM 1 4.0 6.0 14.5 75.5 1.3 11.2 1.1 7.1
NBM 2 5.8 10.5 19.3 64.3 2.8 7.4 1.7 13.0
NBM 3 5.5 7.6 14.1 72.7 0.5 6.2 0.3 16.0
Mean ± SEM 5.1 ± 0.6 8.0 ± 1.3 16.0 ± 1.7 70.9 ± 3.3 1.5 ± 0.7 8.3 ± 1.5 1.0 ± 0.4 12.0 ± 2.6
CML 1 3.9 7.2 13.4 75.5 2.9 11.1 2.1 7.1
CML 2 2.0 3.1 16.1 78.7 3.7 26.8 5.5 16.9
CML 3 2.0 3.1 12.7 82.3 0.5 10.9 0.5 6.7
CML 4a 1.3 2.1 12.3 84.3 3.3 17.6 2.1 15.6.
CML 5 2.2 9.5 12.8 75.5 5.4 7.1 4.5 6.4
Mean ± SEM 2.3 ± 0.4* 5.0 ± 1.4 13.5 ± 0.7 79.3 ± 1.8* 3.2 ± 0.8 14.7 ± 3.5 2.9 ± 0.9 10.5 ± 2.3
  1. Cell cycle and Rhodamine 123 (Rh-123) staining was performed and analyzed as described in Fig. 1. Results are shown as the individual data profiles and the mean ± standard error of the mean (SEM) for each population. A Students t-test was applied to all the data and significant differences between normal and CML samples are marked (* p < 0.05). aCML4 is data from a sample of bone marrow.