Fig. 3

AGE-BSA stimulates phosphorylation of transcription factors. Quiescent confluent cardiac primary fibroblasts from the adult rat were incubated with 25 µM AGE-BSA or BSA for the indicated times. Activation of transcription factors ATF-2 and c-jun was analyzed by immunoblotting using phosphospecific antibodies. Cardiac fibroblasts were stimulated as described in Figure 2. For analysis, phosphospecific antibodies representing activation sites for the transcription-factors ATF-2 and c-jun were used.