Table 1 Primers used for RT-PCR

Collagen 1 (52)

se: CCC ACG TAG GTG TCC TAA AGT

as: CCG TGG TGC TAA AAT AAT AAA

346 bp (1012–1358)

55/30

KO1832

Collagen 3 (52)

se: CGAGGTAACAGAGGTGAAAGA

as: AACCCAGTATTCTCCGCTCTT

336 bp

(797–1132)

55/30

AJ005395

Collagen 4 (53)

se: GGGAGCATGAAGGGACAG

as: CAGGGCCTTGCTGGCTTC

393 bp (2961–3353)

55/30

J04694

Fibronectin (54)

se: GAGAGATCTGGAGGTCAT

as: GGGTGACACCTGAGTGAA

853 bp

(4830–5682)

55/22

X15906

Laminin

se: CCACCTCTGAACACCAAGGC

as: TGTTCTCTGGGGCTGATGGT

413 bp

(4420–4832)

55/30

U61261

MMP-2

se: AGCTCATCGCAGACTCCTGG

as: CAGCACCTTTCTTTGGGCAC

362 bp (2426–2787)

55/22

X71466

MMP-7

se: TTTGATGGGCCAGGAAACAC as: TGTGCAAAACCCATCCACAG

398 bp (574–971)

55/30

L24374

MMP-9

se: CTACTGGCACACGCCTTTCC as: CCGAGCGACCTTTAGTGGTG

385 bp (589–973)

55/30

U36476

MMP-13

se: ACCTGGACAAGCAGCTCCAA as: CAGGGCCCAGAATTTTCTCC

456 bp

(602–1057)

55/35

M60616

MT-MMP

(MMP-14)

se: CAAGTCAGC^^TCTGCGGGACT

as: CAGGATGGGTGAGAACAGCC

379 bp

(1749–2127)

55/30

X91785

TIMP-I

se: CATGGGTTCCCCAGAAATCA

as: AGTTTGCAAGGGATGGCTGA

366 bp

(162–527)

55/22

U06179

TIMP-2 (55)

se: CAAAGGACCTGACAAGGAC

as: TTGATGCAGGCAAAGAAC

348 bp

(445–792)

55/25

L31884

TGF-beta 1 (56)

se: ACGTCAGACATTCGGGAAGCAGTG

as: GCAAGGACCTTGCTGTACTGTGTG

611 bp

(824–1434)

55/28

X52498

GAPDH

se: TGGAAAGCTGTGGCGTGATG

as: TCCACCACCCTGTTGCTGTAGC

402 bp

(600–1001)

55/20

M17701