Fig. 4
From: VEGF-D is an X-linked/AP-1 Regulated Putative Onco-angiogen in Human Glioblastoma Multiforme
VEGF-D and FRA-1 in different stages of malignancy or in growth factor-stimulated cancer cells. (A) Western blot immunoreactivity for VEGF-D in Fra-1 (-) or Fra-1 (+) cells. Equal amounts (100 µg/lane) of cell lysate proteins derived from CSML0 (low Fra-1), CSML100 (high Fra-1), CSMLO pMV fra-1 (-) (mock-transfected CSML0), and CSMLO pMV fra-1 (+) (fra-1-transfected CSML0) cell lines were electrophoretically separated and blotted onto a PVDF membrane. A positive control lysate from mouse heart was included in the analysis. The immunoreactivity was developed using chemiluminescence. (B) Co-staining for Fra-1 (red) and VEGF-D (green) in SNB-19 cells stimulated with EGF or LIF for 24 hr.