Differential Susceptibility of Naïve and Activated Human γδ T Cells to Activation-Induced Cell Death by T-Cell Receptor Cross-Linking

Table 1 mRNA expression of cytokines, Fas, FasL and FLIP of naïve and activated γδ T cells after TCR cross-linking

	Fas			FasL			FLIP			IL-2			IL-4			TNF α		IFN γ
Time (h)	N	A	CsA	N	A	CsA	N	A	CsA	N	A	CsA	N	A	CsA	N	A	N	A
0	−−	+ +	++	++	++	++	+−	−−	−−	−−	−−	−−	−−	+−	++	++	−−	−−	++
4	−−	++	++	++	++	++	++	−−	++	−−	++	−−	−−	++	++	++	++	−−	++
8	−−	++	++	+−	++	++	−−	−−	−−	−−	−−	−−	−−	+−	−−	+−	−−	−−	++
12	−−	++	++	+−	++	++	+−	−−	−−	−−	−−	−−	−−	−−	−−	+	−−	−−	−−
24	ND	−	ND	−	ND	+	+	−	+	−	−	−	−	−	−	−	−	ND	−

mRNA expression was examined by RT-PCR for naïve (N), activated (A) and CsA treated γδ T cells at various time-points after stimulation through MACS. The data set for each time-point for both naïve and activated cells represents two sets of experiments from different cultures, where + denotes the presence and-the absence of the mRNA in question. The “+/−” indicates the situation, where one experimental data set showed the presence of the given mRNA, whereas the other set showed its absence. If both experiments were positive or negative, it is denoted as ++ or −−, respectively. ND denotes not determined as several samples at t24 showed degradation of RNA and did not show a consistent signal for β-actin amplification. The rest of the data for t24 is from one experiment.

ISSN: 1528-3658