Fig. 8

FAS increases and pro-caspase-8 decreases following neonatal H-I. (A) Twenty four hours following H-I at P7, hippocampal lysates from bax +/−, and −/− mice (30 µg protein per lane) from the undamaged-right (R) and damaged-left (L) hemisphere were assessed by SDS-PAGE followed by Western blotting with antibodies to FAS, pro-caspase-8 and β-actin. There was a clear increase in FAS (∼45 kDa) and a decrease in pro-caspase-8 (∼57 kDa) in all types of mice assessed. (B) The density of the band corresponding to full length caspase-8 was determined in the right and left hippocampi of groups of bax +/− and bax −/− mice corresponding to the lanes shown above in A. There was no statistical difference in the amount of caspase-8 24 hours following H-I when comparing the injured (L) hippocampi between bax +/− to bax −/− mice (N = 4 per group).