Identification of apoptotic oligodendrocytes and IFN
-positive cells at the margins of chronic active MS lesions
Cryostat sections of fresh frozen tissue from MS patients (17) were processed for colabeling apoptotic nuclei (brown) and MOG (purple/black), (a and b), human IFNγ (d and e), and oil-O-red to identify active phagocytic cells (c). Panels a–d show a chronic active MS lesion, whereas Panel e shows a region of unaffected white matter from the same patient. The tissue was removed at autopsy performed 4 hr after death and frozen. Some freezing artifacts are apparent on the sections.