Fig. 2
From: Compounds That Target Novel Cellular Components Involved in HIV-1 Transcription
Analyses of HIV-1 and cellular gene transcription in the presence of benzothiophene derivatives
(a) OM-10.1 cultures were harvested after 2 hr (for autocrine TNFα) or 24 hr (for HIV-1) of TNFα treatment and total cellular RNA was hybridized with specific probes. Molecular size indicators depict the three well-recognized HIV-1 transcripts. Ethidium bromide-stained 28S ribosomal RNA is shown to verify the quantity and integrity of each sample, (b) HIV-1 transcription after TNFα induction of U1 promocytes and in constitutively expressing 8E5 cultures (c) PMA-induced HIV-1 transcription (upper) and cellular differentiation (lower) in OM-10.1 promyelocytes. Macrophage-like differentiation was determined by flow cytometric analysis of Mo3e surface expression before (— · —) and 24 hr after PMA treatment (—) and 24 hr after PMA treatment in the presence of PD 121871 (— —) or PD 144795 (- - - -). Data are representative of more than five separate experiments.