Expression of V3-23 and V3-21 transcripts in unstimulated peripheral B cells of normals and RA patients
Peripheral blood lymphocytes were isolated from 10 normal donors and 10 RA patients. cDNA was amplified with VH3 and IgM specific primers. Amplified products were adjusted for equal amounts of IgM transcripts, dot blotted onto nitrocellulose membranes, and hybridized with biotinylated oligonucleotide probes specific for V3-21 or V3-23. The binding of the oligonucleotides was semiquantified using an Ambis system. Average densities of the V3-23 and V3-21 probes bound to VH3-specific transcripts from normal donors and RA patients are given.